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Validated Methods for Inactivation of Tick-Borne Encephalitis Virus Compatible with Immune-Based and Enzymatic
Simone Leoni1,2,3, Stephen L Leib1,2, Katharina Summermatter1
1Institute for Infectious Diseases, University of Bern, 3001 Bern, Switzerland.
Viruses
|June 27, 2025
Summary
Effective inactivation of Tick-Borne Encephalitis Virus (TBEV) is crucial for research. UV irradiation and Triton-X100 successfully inactivated TBEV while preserving sample integrity for downstream assays.
Area of Science:
- Virology
- Public Health
- Biosafety
Background:
- Tick-Borne Encephalitis Virus (TBEV) poses a growing public health concern in Eurasia.
- TBEV is a risk group 3 organism in Switzerland, requiring specialized BSL-3 laboratory handling.
- Research expansion necessitates safe methods for processing TBEV samples outside high-containment facilities.
Purpose of the Study:
- To evaluate inactivation methods for TBEV.
- To ensure processed samples remain compatible with downstream analytical assays.
- To establish safe protocols for TBEV research beyond BSL-3 containment.
Main Methods:
- Inactivation of TBEV in culture supernatants and tissue homogenates.
- Testing UV irradiation, chemical treatment (Triton-X100), and mechanical filtration.
- Assessing sample integrity for immuno- and enzymatic assays post-inactivation.
Main Results:
- UV irradiation (45 seconds) effectively inactivated TBEV.
- Triton-X100 at 0.05%-0.1% concentrations inactivated TBEV.
- Both methods largely preserved sample integrity for downstream analyses.
Conclusions:
- UV irradiation and Triton-X100 are effective and compatible methods for TBEV inactivation.
- These protocols enable safe TBEV research outside of high-containment laboratories.
- Validated inactivation procedures are essential for advancing TBEV research infrastructure.
