Related Experiment Video
Updated: Sep 18, 2025
10:16
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
Published on: August 16, 2024
1.4K
Recent Advances in Aptamer-Based Point-of-Care Testing
Senlin Luo1, Xiaotian Zhang2, Yuzhuo Zhang2
1Department of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Sensors (Basel, Switzerland)
|June 27, 2025
Summary
Aptamers offer rapid, accurate point-of-care diagnostic tests by selectively binding molecular targets. This review highlights advances in aptamer-based sensors, challenges, and future directions in diagnostics.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Molecular Diagnostics
Background:
- Aptamers are oligonucleotide probes with high specificity for molecular targets.
- Their versatility enables diverse applications in diagnostics.
- Aptamers offer advantages for point-of-care testing (POCT).
Purpose of the Study:
- To review recent advances in aptamer-based POCT.
- To highlight innovative applications in various sensor types.
- To discuss challenges and future perspectives.
Main Methods:
- Comprehensive literature review of aptamer-based POCT.
- Focus on colorimetric sensors, lateral flow assays, fluorescent biosensors, and electrochemical biosensors.
- Analysis of current challenges and future outlook.
Main Results:
- Aptamers show significant promise for rapid and accurate POCT.
- Innovative applications demonstrated across multiple sensor platforms.
- Key challenges identified in aptamer selection, stability, and assay integration.
Conclusions:
- Aptamer-based POCT is a rapidly advancing field with significant potential.
- Further research is needed to overcome existing challenges.
- Future perspectives include improved aptamer design and multiplexed detection for enhanced diagnostics.
