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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 17, 2026

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

Published on: May 2, 2016

Engineered Lipid Coronas for Gold Nanoparticle-Based Bioorthogonal Catalysis.

Aarohi Gupta¹, Avijit Maity^1,2, William Ndugire¹

¹Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.

|June 27, 2025

Summary

This summary is machine-generated.

Researchers developed lipid-coated gold nanoparticles (AuNPs) for robust bioorthogonal catalysis. This innovation protects catalysts in biological settings, enabling effective prodrug activation in cancer cells.

Keywords:

bioorthogonal catalysis gold nanoparticles lipid corona uncaging

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Area of Science:

Biomedical Engineering
Catalysis
Nanotechnology

Background:

Bioorthogonal catalysis offers precise 'turn-on' activation of prodrugs and pro-dyes.
Gold nanoparticle (AuNP) catalysts are selective and nontoxic but vulnerable to deactivation by biological molecules.
Protecting AuNPs is crucial for their application in complex biological environments.

Purpose of the Study:

To develop a protective strategy for AuNP catalysts to enable bioorthogonal catalysis in vivo.
To investigate the role of a lipid corona in stabilizing AuNPs against biological deactivation.
To demonstrate the efficacy of lipid-coated AuNPs in activating prodrugs within cancer cells.

Main Methods:

Synthesized gold nanoparticles (AuNPs) and coated them with a modular lipid corona.
Characterized the lipid corona's influence on surface charge, membrane fluidity, and PEG content.
Assessed the stability and catalytic activity of lipid-coated AuNPs in biological conditions.
Demonstrated prodrug activation using lipid-coated AuNPs in cancer cells.

Main Results:

Lipid coating effectively protected AuNP catalysts from deactivation by serum proteins and thiols.
The modular lipid corona allowed for tunable surface properties.
Lipid-coated AuNPs successfully converted inactive propargylated doxorubicin to its active form in cancer cells.
Demonstrated the potential of this system for targeted drug delivery and activation.

Conclusions:

Lipid-coated AuNPs represent a significant advancement for bioorthogonal catalysis in biomedical applications.
This strategy enhances catalyst stability and activity in complex biological media.
The system shows promise for controlled 'turn-on' drug activation within cancer cells, offering a new therapeutic approach.