Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distribution and functional significance of rodent cerebellar glycogen.

iScience·2026
Same author

Towards enhancing photostability and adeno-associated viral vector delivery for genetically encoded plasma fluorescent labels.

Neurophotonics·2025
Same author

Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP<sub>3</sub>R2-mediated Ca<sup>2+</sup> signaling in mice.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Functional and structural profiling of circulation via genetically encoded modular fluorescent probes.

bioRxiv : the preprint server for biology·2025
Same author

Brain State-Dependent Neocortico-Hippocampal Network Dynamics Are Modulated by Postnatal Stimuli.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025
Same author

Oxygen imaging of hypoxic pockets in the mouse cerebral cortex.

Science (New York, N.Y.)·2024

Related Experiment Video

Updated: Jun 18, 2026

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo
14:22

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Published on: November 20, 2021

3.8K

Genetic tools for imaging microcirculation via plasma labeling.

Xiaowen Wang1, Marta Vittani1, Ashley Bomin Lee1

  • 1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Anatomical Science International
|June 27, 2025
PubMed
Summary
This summary is machine-generated.

New genetic techniques using adeno-associated virus (AAV) enable stable, long-term imaging of cerebral microcirculation. These methods offer a significant advancement for studying brain function and disease in mouse models.

Keywords:
AAV-mediated knock-inAdeno-associated virusAlbuminCRISPR-Cas9Fluorescence imagingFluorescent protein

More Related Videos

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.5K
A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
10:39

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

Published on: May 24, 2022

2.5K

Related Experiment Videos

Last Updated: Jun 18, 2026

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo
14:22

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Published on: November 20, 2021

3.8K
Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.5K
A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
10:39

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

Published on: May 24, 2022

2.5K

Area of Science:

  • Neuroscience
  • Vascular Biology
  • Molecular Imaging

Background:

  • Cerebral microcirculation is vital for brain function, supplying nutrients and removing waste.
  • Disruptions in brain blood flow are linked to neurodegenerative diseases, stroke, and cognitive decline.
  • Current imaging methods like fluorescent dextran are limited for long-term studies.

Purpose of the Study:

  • To introduce novel molecular genetic techniques for stable, long-term cerebral microcirculation imaging.
  • To highlight the advantages of adeno-associated virus (AAV)-mediated approaches for longitudinal studies.

Main Methods:

  • Utilizing recombinant adeno-associated virus (AAV) for plasma label expression.
  • Employing genomic knock-in strategies for stable label integration.
  • Single systemic injection for simplified administration in mouse models.

Main Results:

  • Developed AAV-mediated and genomic knock-in methods for stable microcirculation imaging.
  • These techniques facilitate longitudinal imaging in disease models.
  • Single injection simplifies experimental procedures.

Conclusions:

  • Novel AAV-mediated genetic techniques enable unprecedented long-term imaging of cerebral microcirculation.
  • These methods are valuable tools for systems biology and understanding brain vascular dynamics.
  • Future applications include detailed studies of neurological diseases and cognitive impairments.