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Study on the synthesis, structural characterization, and anti-hepatoma cell proliferation effects of sulfated alfalfa
Lang Qiu1, Jia-Yi Wang1, Dan-Qi Li2,3
1College of Chemical Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China.
Abstract:
A water-soluble polysaccharide (APS) from Medicago Sativa L. (alfalfa) stems was sulfated via the chlorosulfonic acid (CSA)-pyridine (Pyr) method under three conditions, yielding SAPS-1, SAPS-2, and SAPS-3. Structural characterization (HPGPC, NMR, FT-IR, SEM) confirmed successful sulfation and increased water solubility. SAPSs 1-3 exhibited superior scavenging of DPPH, ABTS, and OH radicals compared to APS. Both APS and SAPSs 1-3 inhibited hepatoma cell (HepG2, Hep3B) proliferation in vitro. Cell cycle analysis showed APS-induced G0/G1 arrest, while SAPS-3 exhibited biphasic regulation by maintaining G0/G1 arrest and stimulating S-phase accumulation. The CSA-Pyr technique enhanced the inhibitory effects, offering promise for developing bioactive compounds from alfalfa biomass.
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