[Effect of thiophosphamide on copper concentration in rabbit tissues]

The copper in different tissues, blood, urine and feces was studied in experiments on rabbits 30 min, 6 h, 1, 3, 7, 15 and 30 days after intravenous administration of thiophosphamide in a dose of 6 mg per 1 kg of weight. The samples of the mentioned tissues were dried and ashed in the muffle furnace. The quantitative determination of copper in ash solution was performed by the diethyldithiocarbamate method using the spectrophotometer Cphi-4A. The thiophosphamide action is shown to cause regular changes in the copper content in the studied tissues of animals in the periods from 6 h up to 30 days inclusive. The occuring shifts are of bilateral character in different periods of the chemical effect, but in most cases a decrease in the copper content in the tissues under study is dominating.