Related Experiment Video

Updated: Sep 17, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.2K

Targeted Amplicon Genotyping by Sanger Sequencing

Anthony Torres1, Reginald Gaudino2,1

  • 1Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 28, 2025
PubMed

Keywords:
AmpliconChromatogram analysisDNAElectrophoresisEnd termination reactionSNP detectionSanger sequencingSingle nucleotide polymorphism (SNP)Targeted sequencing

More Related Videos

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.7K
Targeted DNA Methylation Analysis by Next-generation Sequencing08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.3K

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
757.9K
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.7K