Neuromesodermal progenitor derived mesenchymal stem cells: A new source for osteogenesis and adipogenesis in vitro

Area of Science:

• Stem cell biology
• Regenerative medicine
• Developmental biology

Background:

• Generating unlimited mesenchymal stem cells (MSCs) from induced pluripotent stem cells (iPSCs) is crucial for clinical therapies.
• Identifying specific progenitor populations aids in refining cell differentiation protocols.

Purpose of the Study:

• To generate MSCs from iPSCs via neuromesodermal progenitors (NMPs) using an in vitro protocol.
• To evaluate the osteogenic and adipogenic differentiation potential of these iPSC-derived MSCs (iMSCs).

Main Methods:

• Established in vitro differentiation protocol for iMSC generation via NMPs.
• In vitro cell culture assays, gene, and protein expression analysis for differentiation potential.
• Membrane array and LC-MS/MS analysis for iMSC protein profile and secretome.

Main Results:

• iMSCs demonstrated both osteogenic and adipogenic differentiation capacity in vitro.
• iMSC protein profiles differed from dental pulp and adipose stem cells.
• iMSC secretome significantly promoted adipogenesis in cultured preadipocytes.

Conclusions:

• MSCs with adipogenic and osteogenic potential can be successfully derived from iPSC-derived NMPs in vitro.
• The secretome of iMSCs exhibits adipogenic potential, offering promising therapeutic avenues.