Bacterial Cellulose-Based Laser-Scribed Graphene Electrode for Hydrogen Peroxide Detection in Cancer Cells

Area of Science:

• Biomedical Engineering
• Materials Science
• Analytical Chemistry

Background:

• Sustainable and high-performance electrochemical sensors are vital for biomedical applications.
• Bacterial cellulose (BC) is a biodegradable and renewable resource.
• Laser-scribed graphene (LSG) offers a flexible and miniaturized electrochemical platform.

Purpose of the Study:

• To develop a novel electrochemical sensor for hydrogen peroxide (H2O2) detection.
• To utilize bacterial cellulose-derived laser-scribed graphene (BC-LSG) modified with MXene and platinum nanoparticles (PtNPs).
• To assess the sensor's performance and applicability in biological systems.

Main Methods:

• Cultivation of bacterial cellulose (BC) and its transformation into BC-LSG via CO2 laser irradiation.
• Modification of BC-LSG with MXene and platinum nanoparticles (PtNPs).
• Electrochemical characterization and H2O2 detection in mammalian cells.

Main Results:

• The BC-LSG/MXene/PtNPs sensor exhibited enhanced electrocatalytic activity towards H2O2 oxidation.
• Achieved a wide linear concentration range (15-95 μmol L-1) and a low detection limit (0.35 μmol L-1).
• Demonstrated superior stability, reproducibility, and eco-friendliness compared to enzymatic sensors.
• Successfully applied for H2O2 detection in mammalian cells for oxidative stress monitoring.

Conclusions:

• The developed sensor is a scalable, disposable, and sustainable electrochemical device.
• It shows significant potential for real-time monitoring of oxidative stress in biological contexts.
• Highlights the synergy of biopolymeric materials, nanotechnology, and laser processing for advanced sensor development.