An integrated analysis of second- and third-generation transcriptome sequencing technologies reveals the DAZAP1...

Area of Science:

• Reproductive Biology
• Molecular Genetics
• Spermatogenesis Research

Background:

• Pig sperm quality is vital for reproductive success.
• Sperm defects impact boar fertility and offspring production.
• The DAZAP1 gene regulates alternative splicing in cellular processes like spermatogenesis.

Purpose of the Study:

• To identify and characterize DAZAP1 gene transcripts in pigs.
• To investigate the role of DAZAP1 in pig spermatogenesis and male reproduction.

Main Methods:

• Second- and third-generation transcriptome sequencing of Banna mini-pig testicular cDNA.
• Identification and analysis of DAZAP1 splice variants.
• Gene Ontology (GO) and KEGG pathway enrichment analyses.
• ceRNA analysis to identify miRNA interactions.
• Multi-tissue expression analysis and subcellular localization.

Main Results:

• Three DAZAP1 splice variants (DAZAP1_X1, DAZAP1_X2, DAZAP1_X3) were identified.
• DAZAP1_X2, with 13 exons and a 1254 bp CDS, is involved in spermatogenesis, ciliary movement, germ cell development, and sexual reproduction.
• DAZAP1_X2 interacts with ssc-miR-107, ssc-miR-127, and ssc-miR-1343, all linked to spermatogenesis.
• DAZAP1 is highly expressed in the testis and urethral bulb and localized to the cell nucleus.
• DAZAP1 is critical for sperm formation and male reproductive function.

Conclusions:

• DAZAP1 plays a critical role in pig sperm formation and male fertility.
• DAZAP1_X2 is a key transcript involved in essential reproductive processes.
• This research elucidates the biological functions of DAZAP1 in pigs, highlighting its importance for reproduction.