Related Experiment Video
Updated: Sep 17, 2025
Activity-based Training on a Treadmill with Spinal Cord Injured Wistar Rats
Published on: January 16, 2019
Transspinal stimulation preceding assisted step training reorganizes neuronal excitability and function of inhibitory
Abdullah M Sayed Ahmad1,2, Morad Zaaya1,2, Noam Y Harel3,4
1Klab4Recovery Research Program, The City University of New York; New York, 10314, USA.
Introduction:
In this pilot randomized sham-controlled clinical trial, we characterized the spinal neuronal and network excitability in human spinal cord injury (SCI) when transspinal stimulation preceded locomotor training within the same session.
Methods:
Fourteen participants with chronic SCI received an average of 40 sessions with 30 Hz transspinal stimulation delivered for 30 minutes during standing (active: n= 4; sham: n= 5) or supine (active: n= 5) followed by 30-minutes of robotic assisted step training. Before and 1-2 days after completion of all training sessions, we assessed the soleus H-reflex homosynaptic depression and soleus H-reflex recruitment curve, and the amount of reciprocal and presynaptic inhibition following conditioning stimulation of the antagonistic common peroneal nerve.
Results:
Transspinal stimulation administered before locomotor training increased the amount of homosynaptic depression in the active-standing and active-supine groups, while presynaptic inhibition exerted on Ia afferent terminals increased in all study groups. Reciprocal Ia inhibition improved in the sham-standing and active-supine groups while in all groups the excitability threshold of muscle group Ia afferents was decreased in all groups.
Conclusion:
This study demonstrated that transspinal stimulation preceding locomotor training partially restores natural spinal inhibition and produces network reorganization in chronic SCI. Noninvasive transspinal stimulation can increase the benefits of locomotor training, bringing spinal neuronal networks to a more functional state in chronic SCI.
