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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

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Related Experiment Video

Updated: Sep 17, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

A Microfluidic Strategy to Capture Antigen-Specific High-Affinity B Cells.

Ahmed M Alhassan¹, Venktesh S Shirure¹, Jean Luo²

¹Department of Biomedical Engineering University of California Davis CA 95616 USA.

Advanced Nanobiomed Research

|June 30, 2025

Summary

This summary is machine-generated.

This study introduces a microfluidic method to measure B cell receptor (BCR) affinity to antigens under force. This approach rapidly identifies high-affinity B cells, aiding immune status assessment and therapeutic applications.

Keywords:

affinities avidities cell separations immunology

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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Last Updated: Sep 17, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Published on: August 22, 2019

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Area of Science:

Immunology
Biotechnology
Microfluidics

Background:

Current methods for assessing B cell responses are costly and time-consuming.
Existing techniques measure antibody affinity in solution, neglecting the influence of force on B cell receptor (BCR)-antigen interactions.
Emerging evidence suggests mechanical force impacts BCR-antigen binding, potentially affecting immune status.

Purpose of the Study:

To develop a microfluidic system for assessing antigen-specific B cell receptor (BCR) binding affinity under varying forces.
To investigate the relationship between applied force, bound lifetime, and B cell affinity.
To establish a rapid and cost-effective method for identifying high-affinity B cells.

Main Methods:

Designed a laminar flow microfluidic chamber with surface-bound influenza A hemagglutinin antigen.
Assessed antigen-specific BCR binding affinity of hybridomas across a force range of 65 to 650 pN.
Correlated microfluidic binding affinity with solution-based antibody affinity measurements.

Main Results:

Demonstrated that increasing shear force and bound lifetime can enrich for antigen-specific, high-affinity B cells.
Showed that the affinity of membrane-bound BCRs in the flow chamber strongly correlates with the affinity of matched antibodies in solution.
Validated the microfluidic strategy for rapid assessment of BCR-antigen binding properties.

Conclusions:

The developed microfluidic strategy offers a rapid method to assess B cell receptor (BCR)-antigen binding properties.
This approach can identify antigen-specific high-affinity B cells, enabling functional immune status assessment.
The technology holds potential for cost-effective identification of B cells for therapeutic antibody development.