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Related Experiment Video

Updated: Sep 17, 2025

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Published on: October 15, 2019

Real-Time Monitoring of DNA Origami-Cell Interactions via Single Particle Tracking.

Indra Van Zundert¹, Elena Spezzani¹, Roger R Brillas¹

¹Biomedical Engineering Department, Technische Universiteit Eindhoven, Het Kranenveld 14, 5612 AZ, Eindhoven, The Netherlands.

Small (Weinheim an Der Bergstrasse, Germany)

|June 30, 2025

Summary

This summary is machine-generated.

DNA origami nanorods functionalized with ligands selectively target cancer cells, revealing real-time molecular interactions. This study enhances understanding of DNA origami behavior at cell interfaces for biomedical applications.

Keywords:

DNA Origami cell‐nanoparticle interactions ligand‐receptor binding dynamics single particle tracking

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Last Updated: Sep 17, 2025

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Published on: September 5, 2019

Area of Science:

Biotechnology
Nanotechnology
Cell Biology

Background:

DNA origami offers precise spatial control for surface functionalization.
Studying dynamic ligand-receptor interactions on cell surfaces requires advanced techniques.
Understanding molecular interaction dynamics at the cell interface is crucial for nanomedicine.

Purpose of the Study:

To investigate real-time DNA origami interactions with cell surfaces in situ.
To explore the targeting capabilities of ligand-functionalized DNA nanorods (NRs).
To analyze the impact of ligand density on DNA origami binding characteristics.

Main Methods:

Utilized single particle tracking (SPT) to capture DNA origami-cell encounters.
Functionalized DNA nanorods (NRs) with antibodies or aptamers targeting the epidermal growth factor receptor (EGFR).
Applied NRs to EGFR-overexpressing cancer cells for interaction studies.

Main Results:

Ligand-coated NRs demonstrated selective binding to EGFR on target cancer cells.
Non-functionalized NRs showed minimal interaction with cells.
Aptamer-decorated NRs exhibited nonlinear binding, while antibody-decorated NRs showed less pronounced effects.

Conclusions:

Provided new mechanistic insights into DNA origami behavior at the cell interface with high spatiotemporal resolution.
Demonstrated the selective targeting of cancer cells by ligand-functionalized DNA nanorods.
Aids in the rational design of ligand-targeted DNA origami for biomedical applications.