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Enzyme-Mediated Dissipative Hybridization Chain Reaction (HCR).

Federica Pedrini1, Luca Capelli2, Alessandro Bertucci2

  • 1Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Rome, Italy.

Angewandte Chemie (International Ed. in English)
|March 25, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new enzyme-mediated method to control DNA polymerization using hybridization chain reaction (HCR). This strategy allows for precisely timed DNA assembly and disassembly, enabling programmable control over molecular processes.

Keywords:
DNA nanostructuresDNA nanotechnologydissipative DNA polymersdissipative HCRsupramolecular chemistry

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Synthetic Biology

Background:

  • Hybridization chain reaction (HCR) is a powerful method for isothermal nucleic acid amplification and assembly.
  • Controlling the dynamics and lifetime of HCR processes remains a challenge for advanced applications.
  • Enzymatic regulation offers precise and tunable control over biochemical reactions.

Purpose of the Study:

  • To develop a novel strategy for enzyme-mediated dissipative control over hybridization chain reaction (HCR).
  • To enable transient and programmable regulation of DNA polymer assembly and disassembly.
  • To demonstrate the utility of this approach in complex molecular systems.

Main Methods:

  • Re-engineering a reversible HCR system to incorporate enzymatic fuel consumption.
  • Utilizing RNA/DNA fuel strands in conjunction with specific enzymes to regulate polymer dynamics.
  • Designing and implementing multi-input dissipative HCR systems and transiently controlled DNA scaffolds.

Main Results:

  • Achieved precise, enzyme-mediated control over the lifetime of HCR processes.
  • Demonstrated multi-cycle regulation with programmable durations.
  • Successfully applied the strategy to a multi-input dissipative HCR system controlled by two enzymes.
  • Showcased transient control of a DNA-based polymeric scaffold functionalized with a light-up aptamer.

Conclusions:

  • The developed strategy provides a novel method for dissipative control of HCR using enzymes.
  • This approach allows for precise temporal programming of DNA assembly and disassembly.
  • The demonstrated applications highlight the potential of enzyme-mediated HCR for sophisticated molecular devices and diagnostics.