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Updated: Mar 27, 2026

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
Published on: February 6, 2020
Enzyme-Mediated Dissipative Hybridization Chain Reaction (HCR)
Federica Pedrini1, Luca Capelli2, Alessandro Bertucci2
1Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Rome, Italy.
None:
We report here a novel strategy to achieve enzyme-mediated dissipative control over hybridization chain reaction (HCR). To achieve this, we have rationally re-engineered a reversible HCR approach so that the assembly and disassembly of DNA polymers can be transiently regulated by RNA/DNA fuel strands in combination with specific enzymatic fuel-consuming units. This strategy enables regulation over multiple cycles with precisely programmable lifetimes. Finally, we demonstrate two applications: a multi-input dissipative HCR simultaneously controlled by two different enzymes, and the transient control of a DNA-based polymeric scaffold functionalized with a light-up aptamer.
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