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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Synthetic DNA Transducers Integrate DNA Repair to CRISPR Signal Transduction.

Neda Bagheri1, Alessandro Bertucci2,3, Rosa Merlo4

  • 1Department of Chemical Science and Technologies, University of Rome, Tor Vergata, Via della Ricerca Scientifica 1, Rome 00133, Italy.

ACS Sensors
|February 11, 2026
PubMed
Summary
This summary is machine-generated.

We developed a novel CRISPR-based diagnostic tool that links DNA repair events to CRISPR-Cas12a activation. This system enables sensitive detection of DNA repair enzyme activity and facilitates drug screening.

Keywords:
CRISPRCas12aDNA repair enzymeDNA transducerinhibitor

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Synthetic Biology

Background:

  • CRISPR diagnostics offer advanced nucleic acid detection.
  • Integrating upstream enzyme activity with CRISPR output is an emerging field.

Purpose of the Study:

  • To develop a synthetic platform coupling DNA repair activity with CRISPR-Cas12a activation.
  • To create a method for detecting DNA repair enzyme activity and enabling drug screening.

Main Methods:

  • Developed a synthetic transduction platform linking base excision repair (BER) events to CRISPR-Cas12a activation.
  • Utilized DNA glycosylases (UDG, hOGG1) and a programmable DNA transducer.
  • Converted enzyme activity into a fluorescence signal via Cas12a collateral cleavage.

Main Results:

  • Achieved rapid, sensitive, and specific lysate-based detection of DNA repair activity.
  • Demonstrated adaptability for high-throughput screening of small molecule inhibitors.
  • Established a general framework for transducing DNA repair into programmable CRISPR output.

Conclusions:

  • The synthetic platform enables direct coupling of DNA repair to CRISPR-Cas12a.
  • This approach facilitates sensitive bioanalytical detection and drug screening.
  • Paves the way for DNA repair-responsive synthetic gene circuits and CRISPR-based drug discovery.