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Zwitterionic Thiophene-Functionalized Sensing Interface Enables Robust Antifouling Electrochemical Analysis.
Jiamei Chen1, Xinwei Hu1, Yang Huang1
1Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China.
Analytical Chemistry
|June 30, 2025
Summary
This study presents a novel conductive and antifouling electrochemical sensor for rapid antibody detection in complex samples. The developed sensor demonstrates high sensitivity and potential for clinical applications without sample purification.
Area of Science:
- Electrochemistry
- Materials Science
- Biomedical Engineering
Background:
- Electrochemical sensing interfaces require efficient electron transfer and resistance to nonspecific adsorption for accurate analysis of complex samples.
- Developing robust sensing platforms is crucial for reliable detection of biomarkers in biological fluids.
Purpose of the Study:
- To synthesize a zwitterionic thiophene (Th-Zw) monomer for creating a conductive and antifouling electrochemical sensor.
- To develop a one-step electrochemical detection strategy for antibodies in unprocessed samples.
Main Methods:
- In situ electrodeposition of Th-Zw, graphene oxide, and antigen molecules onto screen-printed electrodes.
- Fabrication of a zwitterionic thiophene-modified electrode for enhanced conductivity and antifouling properties.
- Electrochemical detection of anti-p53 antibody and anti-COVID-19 IgG in spiked human plasma and blood samples.
Main Results:
- The Th-Zw-modified electrode exhibited protein-resistant hydrophobicity, enhanced conductivity, and stable antigen immobilization.
- Achieved low detection limits of 5.95 ng/mL for anti-p53 and 6.58 ng/mL for anti-COVID-19 IgG.
- Demonstrated significant differences in anti-p53 detection between cancer patients and healthy volunteers, indicating clinical applicability.
Conclusions:
- A novel conductive and antifouling electrochemical sensor was successfully developed.
- The sensor enables rapid, sensitive, and label-free detection of antibodies in actual samples, including human plasma and blood.
- This strategy offers a promising new approach for one-step electrochemical detection in clinical diagnostics.
