Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Focusing of Light in the Eye01:16

Focusing of Light in the Eye

3.3K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
3.3K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

6.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
6.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoacoustic tomography using thickness-corrected Fabry-Perot sensors and parallelized camera-based signal acquisition.

Optics express·2026
Same author

A-eye: Automated 3D MRI segmentation and morphometric feature extraction for eye and orbit atlas construction.

PloS one·2026
Same author

Large-area 3D reconstruction of corneal tissues from oscillating focus confocal microscopy.

Scientific reports·2026
Same author

Development and Validation of a Disabling-Glare Mapping Device for Quantifying Dysphotopsia-Related Effects.

Current eye research·2026
Same author

CT Radiomic Features of the Crystalline Lens and Association with Age, Hypertension and Cerebral White Matter Lesions.

Diagnostics (Basel, Switzerland)·2026
Same author

Metamaterial Antennas Enhance MRI of the Eye and Occipital Brain.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: Sep 17, 2025

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses
06:55

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Published on: June 6, 2017

7.7K

Assessment of Diffractive Intraocular Lenses Using Near-Infrared Light: A Critical Evaluation.

Ricardo Elsner1,2, Mario Gerlach3, Sebastian Bohn1,2

  • 1Department of Ophthalmology, Rostock University Medical Center, Rostock, Germany.

Translational Vision Science & Technology
|June 30, 2025
PubMed
Summary

Near-infrared light inaccurately assesses diffractive intraocular lenses (IOLs) after cataract surgery. Visible light is crucial for accurate vision quality assessment of these advanced IOLs.

More Related Videos

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

11.5K
Comparison of Agreement and Accuracy using Binocular Wavefront Optometer with Autorefractor and Phoropter
05:14

Comparison of Agreement and Accuracy using Binocular Wavefront Optometer with Autorefractor and Phoropter

Published on: September 16, 2025

1

Related Experiment Videos

Last Updated: Sep 17, 2025

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses
06:55

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Published on: June 6, 2017

7.7K
Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
07:22

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

11.5K
Comparison of Agreement and Accuracy using Binocular Wavefront Optometer with Autorefractor and Phoropter
05:14

Comparison of Agreement and Accuracy using Binocular Wavefront Optometer with Autorefractor and Phoropter

Published on: September 16, 2025

1

Area of Science:

  • Ophthalmology
  • Optical Engineering
  • Materials Science

Background:

  • Near-infrared (NIR) optical devices are common for in vivo vision quality assessment post-cataract surgery.
  • Evaluating diffractive intraocular lenses (IOLs) requires understanding their optical performance under different illumination conditions.
  • Challenges exist in accurately assessing IOLs with NIR-based devices due to wavelength-dependent optical properties.

Purpose of the Study:

  • Investigate the diffraction efficiencies and orders of a specific trifocal IOL (AT LISA tri 839MP) under visible and NIR illumination.
  • Highlight the limitations of NIR-based devices in evaluating diffractive IOLs.
  • Compare in vitro optical bench results with optics simulations using theoretical diffractive surface topography.

Main Methods:

  • Utilized an optical bench system to generate in vitro through-focus intensity data.
  • Performed simulations using the manufacturer's theoretically designed diffractive surface topography.
  • Compared optical performance under both visible and NIR light illumination.

Main Results:

  • Visible light illumination showed expected through-focus curve intensities and energy efficiencies.
  • NIR illumination resulted in significantly reduced intensities for near and intermediate foci compared to the far focus.
  • Energy efficiency measurements indicated suboptimal performance of near and intermediate foci under NIR light, rendering the IOL almost monofocal.

Conclusions:

  • Diffractive IOL performance is wavelength-dependent, with significant differences observed between visible and NIR light.
  • NIR-based vision assessment devices risk inaccurate evaluations of diffractive IOLs.
  • Accurate assessment necessitates visible-light-based techniques and precise surface topography data in simulations.