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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Sep 17, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry.

Zoi Laspa¹, Anne-Katrin Rohlfing², Meinrad P Gawaz³

¹Department of Cardiology and Angiology, University Hospital Tübingen, University of Tübingen; zoi.laspa@med.uni-tuebingen.de.

Journal of Visualized Experiments : Jove

|June 30, 2025

Summary

This study introduces a 10-color flow cytometry assay to precisely identify platelet subtypes. This method helps link specific platelet functions to distinct subpopulations, aiding cardiovascular disease research.

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Last Updated: Sep 17, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Area of Science:

Hematology
Immunology
Cardiovascular Research

Background:

Platelets (thrombocytes) are vital for hemostasis and thrombosis.
Platelet dysfunction contributes to bleeding and thrombotic disorders, especially in cardiovascular diseases.
Phenotypic characterization is key to linking platelet subpopulations to specific functions.

Purpose of the Study:

To develop and validate a multi-color flow cytometry assay for detailed platelet phenotyping.
To differentiate agonist-specific platelet subtypes beyond the classically defined ones.
To enable precise assignment of platelet subpopulations to distinct functions.

Main Methods:

Established a 10-color flow cytometry panel using specific antibodies and dyes.
Incubated isolated human platelets with agonists and stained them.
Analyzed changes in surface receptor expression and cell viability.

Main Results:

The assay successfully identified distinct platelet subtypes based on agonist stimulation.
Combined markers for activation (CD62P, CD63, PAC-1), inflammation (CXCR4, ACKR3), and apoptosis (Annexin V, Zombie NIR) were utilized.
Demonstrated the ability to define novel, functionally relevant platelet subpopulations.

Conclusions:

The 10-color flow cytometry panel provides a robust method for detailed platelet subtyping.
This approach can reveal new platelet subtypes associated with specific functions in health and disease.
The assay is applicable to basic research, disease models, and patient studies in platelet physiology and pathology.