Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.8K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The complete chloroplast genome sequence and phylogenetic position of <i>Causonis japonica</i> (Thunb.) Raf. (Vitaceae).

Mitochondrial DNA. Part B, Resources·2026
Same author

Microring resonator-assisted Fourier transform spectrometer using thin-film lithium niobate.

Optics express·2026
Same author

Disturbance Observer-Based Model Predictive Control for Multi-Frequency Interference Suppression in Space Laser Communication Systems.

Sensors (Basel, Switzerland)·2026
Same author

Progress on Electrochemical Sensing of Pharmaceutical Drugs in Complex Biofluids.

Chemosensors (Basel, Switzerland)·2026
Same author

Structural color based on a TiO<sub>2</sub> semi-ellipsoidal nanostructures metasurface.

Optics express·2026
Same author

Broadband achromatic silicon metalens integrated with silicon nitride waveguide across the RGB spectrum.

Optics express·2026

Related Experiment Video

Updated: Sep 17, 2025

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
08:21

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Published on: September 15, 2021

2.3K

A Differential Impedance Flow Cytometry Front-End With Baseline Current Cancellation.

Siyuan Yu, Louis Marun, Matthew L Johnston

    IEEE Transactions on Biomedical Circuits and Systems
    |July 1, 2025
    PubMed
    Summary
    This summary is machine-generated.

    We developed a power-efficient analog front-end (AFE) circuit for impedance flow cytometry. This circuit enhances signal detection for small particles, improving flow cytometry performance.

    More Related Videos

    Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
    06:22

    Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

    Published on: January 9, 2019

    13.2K
    Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
    10:01

    Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

    Published on: December 16, 2022

    4.3K

    Related Experiment Videos

    Last Updated: Sep 17, 2025

    High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
    08:21

    High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

    Published on: September 15, 2021

    2.3K
    Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
    06:22

    Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

    Published on: January 9, 2019

    13.2K
    Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
    10:01

    Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

    Published on: December 16, 2022

    4.3K

    Area of Science:

    • Electrical Engineering
    • Biomedical Engineering
    • Microfluidics

    Background:

    • Impedance-based flow cytometry requires sensitive readout circuits to detect cellular or particle properties.
    • Existing analog front-end (AFE) circuits often face challenges with baseline current, TIA saturation, and noise, limiting performance.
    • A robust AFE is crucial for accurate and efficient particle analysis in microfluidic systems.

    Purpose of the Study:

    • To design and demonstrate a high-performance analog front-end (AFE) circuit for impedance-based flow cytometry.
    • To address limitations of existing AFEs, such as TIA saturation and noise, through innovative circuit design.
    • To validate the AFE's capability for detecting small particles in a microfluidic flow cell.

    Main Methods:

    • Designed a novel AFE circuit using a 180nm CMOS process, featuring a digitally tunable and calibrated cancellation current path.
    • Interfaced the AFE with a three-electrode sensor topology for center electrode excitation and differential current output.
    • Implemented digital calibration using an off-chip ADC and automated algorithm for precise operation.
    • Characterized the AFE's performance, including input-referred current noise density and excitation frequency range.

    Main Results:

    • Achieved a low input-referred current noise density of $1.7 \text{pA}/\sqrt{\text{Hz}}$ at 1MHz excitation with floating inputs.
    • Demonstrated detection of 3um diameter particles in a microfluidic flow cell, confirming practical utility.
    • Operated across an excitation frequency range of 0.5MHz to 10MHz with a power consumption of 15.6mW.
    • The cancellation current path effectively prevented TIA saturation, enabling higher gain and improved noise rejection.

    Conclusions:

    • The developed AFE circuit offers high performance and power efficiency for impedance-based flow cytometry.
    • The digitally tunable cancellation current path is key to overcoming TIA saturation and enhancing signal integrity.
    • The AFE's demonstrated particle detection capability validates its suitability for advanced microfluidic diagnostic applications.