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Simple, effective method for purifying the AS-1 cyanophage
Applied and Environmental Microbiology
|April 1, 1977
Summary
Researchers purified the infectious AS-1 virus using bentonite treatment, rotary evaporation, and density gradient centrifugation. This method efficiently isolated the highly infectious AS-1 virus for further study.
Area of Science:
- Virology
- Biochemistry
Background:
- The AS-1 virus is known for its high infectiousness.
- Efficient purification methods are crucial for virological research.
Purpose of the Study:
- To describe a method for purifying the highly infectious AS-1 virus.
Main Methods:
- Virus-induced culture lysates were treated with bentonite.
- Concentration was achieved through rotary evaporation.
- Density gradient centrifugation was employed for final purification.
Main Results:
- Highly infectious AS-1 virus was successfully purified.
- The purification process involved multiple biophysical techniques.
Conclusions:
- The described method provides a means to obtain purified, highly infectious AS-1 virus.
- This purified virus can be utilized in subsequent research applications.