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Updated: Sep 17, 2025
09:38
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
Published on: February 14, 2017
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A non-binding surface demonstrates increased thrombogenicity under plasma-free conditions.
Cezary Watala1, Boguslawa Luzak1, Tomasz Przygodzki1
1Department of Haemostasis and Haemostatic Disorders, Chair of Biomedical Sciences, Medical University of Lodz, ul. Mazowiecka 6/8, Lodz, 92-215, Poland.
Scientific Reports
|July 1, 2025
Summary
Commercially available non-binding microplates show variable thrombogenicity for platelet function studies. Their performance depends on the presence of plasma, suggesting potential use in specific in vitro platelet assays.
Area of Science:
- Biomaterials Science
- Hematology
- Translational Research
Background:
- Commercially available non-binding microplates are characterized as low-fouling materials.
- Their suitability for studying platelet function in basic research remains undetermined.
- Thrombogenicity assessment is crucial for selecting appropriate materials for platelet assays.
Purpose of the Study:
- To compare the thrombogenicity of non-binding microplates with polypropylene (PP) and polystyrene (PS) microplates.
- To evaluate the suitability of non-binding surfaces for platelet function assays.
- To investigate the influence of plasma components on platelet adhesion to different polymer surfaces.
Main Methods:
- Platelet assays were performed using platelet-rich plasma (PRP) and isolated platelets from healthy donors.
- Thrombogenicity was assessed on non-binding, PP, and PS microplate surfaces.
- Platelet adhesion and deposition were quantified under varying experimental conditions.
Main Results:
- Non-binding surfaces showed similar or lower thrombogenicity than PP and PS in PRP.
- With isolated platelets, non-binding surfaces exhibited similar or higher thrombogenicity.
- A significant asymmetry in platelet adhesion was observed, dependent on plasma presence.
Conclusions:
- Non-binding surfaces may interact directly with platelets, influencing adhesion.
- The non-binding microplate can be a viable alternative for in vitro platelet assays when plasma is present.
- Material choice is critical for accurate platelet function assessment, especially regarding plasma component influence.
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