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[The Autobac MTS system].

G Picerno, G Monticone, R Sconfienza

    Quaderni Sclavo Di Diagnostica Clinica E Di Laboratorio
    |March 1, 1985
    PubMed
    Summary
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    The Autobac MTS system offers precise Minimum Inhibitory Concentration (MIC) determination for urine cultures using turbidimetry. However, its aminoglycoside MIC results showed some disagreement with existing literature.

    Area of Science:

    • Clinical Microbiology
    • Infectious Diseases
    • Diagnostic Technology

    Background:

    • The Autobac MTS is a semiautomatic system for urine culture screening, antibiogram, and Minimum Inhibitory Concentration (MIC) testing.
    • The system utilizes turbidimetry as its core testing principle.
    • Accurate MIC determination is crucial for effective antibiotic selection in treating urinary tract infections.

    Purpose of the Study:

    • To critically evaluate the Minimum Inhibitory Concentration (MIC) determination method employed by the Autobac MTS.
    • To compare the precision of the Autobac MTS MIC method with the traditional break-point method.
    • To assess the agreement of Autobac MTS MIC results with established literature for aminoglycoside antibiotics.

    Main Methods:

    • Detailed examination of the Autobac MTS MIC determination, focusing on the regression lines equation.

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  • Standardization of inoculum as a key factor in the Autobac MTS method.
  • Comparative analysis against the break-point method for precision assessment.

    • Main Results:

    • The Autobac MTS method demonstrates superior precision compared to the break-point method, largely attributed to standardized inoculum.
    • Turbidimetric MIC determination using regression analysis was thoroughly investigated.
    • A consistent disagreement between Autobac MTS MIC results and literature values was observed for tested aminoglycosides.

    Conclusions:

    • The Autobac MTS offers a precise method for MIC determination in urine cultures.
    • Standardized inoculum is a significant advantage contributing to the system's precision.
    • Further investigation is warranted regarding the observed discrepancies in aminoglycoside MIC values compared to literature standards.