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Updated: Aug 3, 2026

Designing a Bio-responsive Robot from DNA Origami
Published on: July 8, 2013
Modulating transformation of DNA origami nanoarray via sequence design
Dongfang Wang1,2, Fiona Cole3, Martina Pfeiffer3
1School of Biomedical Engineering, University of Science and Technology of China, Hefei, China.
Abstract:
The four-way DNA junction is the most prevalent structural motif in DNA nanotechnology. Recently, a reconfigurable DNA nanoarray (domino array) was created with this basic motif to realize intricate, stepwise transformation by the information relay between neighboring four-way junction units. Here, we generate a DNA domino array with same sequences at every junction, and use it as a platform to study how the design of DNA bases at junctions influences the kinetics and thermodynamics of transformation of four-way junctions in reconfigurable DNA nanoarrays. By regulating the energy difference and thus the conversion between the two configurations of four-way junctions, we show the transformation of DNA nanoarray can be modulated in a designable manner. The coordinated transformation of four-way junctions in the DNA domino array enables a detailed investigation on array transformation by using Atomic Force Microscopy (AFM) imaging and single-molecule Förster resonance energy transfer (FRET) microscopy.
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