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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Related Experiment Video

Updated: Sep 17, 2025

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye

Published on: January 14, 2021

Spatial Transcriptomic Analyses of Spermatogenesis.

Ndifereke Uboh¹, Sean Vargas², Victoria D Diaz¹

¹Department of Neuroscience, Developmental and Regenerative Biology, The University of Texas at San Antonio, San Antonio, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 2, 2025

Summary

This summary is machine-generated.

Spatial transcriptomics reveals cell variations in testes, overcoming limitations of traditional methods. This study details applying Seeker (SlideSeq V2) for spatial gene expression analysis in mouse testes.

Keywords:

Bioinformatics RNA-seq Seurat Spatial transcriptomics tissue sections

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Last Updated: Sep 17, 2025

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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

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Published on: February 6, 2018

Area of Science:

Molecular Biology
Genomics
Developmental Biology

Background:

Traditional transcriptomics is destructive and lacks spatial context.
Spatial transcriptomics preserves tissue architecture for cellular heterogeneity analysis.
Spermatogenesis exhibits spatial variation crucial for interpreting gene expression data.

Purpose of the Study:

To provide an overview of spatial transcriptome technologies.
To offer guidelines for applying Seeker (SlideSeq V2) spatial transcriptomics to the testis.
To demonstrate bioinformatic analysis of spatial transcriptomic data in mouse testes.

Main Methods:

Overview of spatial transcriptome technologies.
Detailed protocol for Seeker (SlideSeq V2) application on testicular tissue.
Bioinformatic analysis pipeline for spatial gene expression data.

Main Results:

Demonstration of spatial gene expression analysis in mouse testes.
Identification of spatial patterns related to spermatogenesis stages.
Exemplary analysis of round spermatids using spatial transcriptomics.

Conclusions:

Spatial transcriptomics is essential for understanding spatially organized biological processes like spermatogenesis.
Seeker (SlideSeq V2) provides a powerful tool for high-resolution spatial gene expression analysis in the testis.
Integrating spatial context is critical for interpreting complex transcriptomic datasets.