Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

314
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
314

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Energy-responsive multi-catalytic nanogenerator hydrogels synergistically enhance diabetic tendon-to-bone healing via the ages/RAGE/PIEZO2 axis.

Biomaterials·2026
Same author

Decadal structural advances of oleanane-type pentacyclic triterpenoids and their pharmacological activities, a review (2015-2025).

Phytochemistry·2026
Same author

Optimizing the management of laparoscopic equipment in low-resource settings: a SAGES White Paper.

Surgical endoscopy·2026
Same author

Spatiotemporal Distribution Characteristics and Driving Factors of Phytoplankton in the Mainstream of the Yellow River (Shandong Section).

Microorganisms·2026
Same author

CortexCAM: A Camera Array Microscope for Cortex-Wide Cellular Imaging in Freely Locomoting Mice.

Research square·2026
Same author

Comprehensive metabolic profiling of Dictamni Cortex in normal and rheumatoid arthritis rats using UHPLC-Q/TOF-MS/MS coupled with polygonal mass defect filtering.

Journal of pharmaceutical and biomedical analysis·2026

Related Experiment Video

Updated: Sep 17, 2025

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
08:57

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

18.9K

Rapid 3D imaging at cellular resolution for digital cytopathology with a multi-camera array scanner (MCAS).

Kanghyun Kim1, Amey Chaware1, Clare B Cook1

  • 1Department of Biomedical Engineering, Duke University, Durham, 27708, NC, USA.

Npj Imaging
|July 3, 2025
PubMed
Summary

A new Multi-Camera Array Scanner (MCAS) rapidly digitizes thick cytology specimens for 3D analysis. Machine learning software assists pathologists by detecting lung adenocarcinoma and classifying lung smears with high accuracy.

More Related Videos

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
07:27

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

7.7K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.1K

Related Experiment Videos

Last Updated: Sep 17, 2025

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
08:57

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

18.9K
Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
07:27

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

7.7K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.1K

Area of Science:

  • Biomedical Engineering
  • Computational Pathology
  • Digital Pathology

Background:

  • Optical microscopy is standard for cytopathology diagnosis.
  • Whole slide scanners are slow, expensive, and not widely available.
  • Cytology specimens require 3D capture due to their large area and thickness.

Purpose of the Study:

  • Introduce a novel parallelized microscope for rapid, wide-field 3D scanning of thick specimens.
  • Develop machine learning software for automated analysis of digitized cytology slides.
  • Enhance diagnostic efficiency and accessibility in cytopathology.

Main Methods:

  • Developed a Multi-Camera Array Scanner (MCAS) with 48 micro-cameras for parallel imaging.
  • Achieved wide fields-of-view (54 × 72 mm²) at high resolutions (1.2 and 0.6 μm).
  • Implemented machine learning models for adenocarcinoma detection and lung smear classification.

Main Results:

  • Digitized entire cytology samples in 3D within minutes.
  • MCAS captures 624 megapixels per snapshot, significantly faster than conventional scanners.
  • Demonstrated a 0.73 recall for adenocarcinoma detection and 0.969 AUC for slide-level classification.

Conclusions:

  • The MCAS system offers a faster, more efficient alternative to conventional whole-slide scanners.
  • Machine learning integration aids pathologists in diagnosing complex cytology specimens.
  • This technology has the potential to improve accessibility and accuracy in cytopathology.