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Updated: Sep 17, 2025
09:57
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
Published on: April 1, 2019
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In situ vascularization and epithelialization of segmental bioengineered trachea based on marrow-derived
Fei Sun1,2,3, Yibo Shan2,3, Shu Pan4
1Department of Thoracic Surgery, The Affiliated Taizhou People's Hospital of Nanjing Medical University, Taizhou, 225300, Jiangsu, China.
Materials Today. Bio
|July 3, 2025
Summary
This study developed a novel biomimetic tracheal graft using 3D printing. The graft, combining poly(ε-caprolactone) and decellularized extracellular matrix, successfully promoted vascularization and epithelialization for tracheal transplantation.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Surgical Innovation
Background:
- Tracheal transplantation requires rapid vascularization and epithelialization for success.
- Current methods face challenges in achieving functional tissue integration.
- Biomimetic scaffolds offer potential for improved graft outcomes.
Purpose of the Study:
- To develop and evaluate a novel biomimetic tracheal graft for in situ transplantation.
- To assess the efficacy of combining endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) for graft integration.
- To investigate the biomechanical and biological properties of a poly(ε-caprolactone)/decellularized tracheal extracellular matrix (PCL/dtECM) scaffold.
Main Methods:
- Isolation and purification of EPCs and MSCs from bone marrow.
- Fabrication of a bilayered PCL/dtECM scaffold using 3D printing.
- Seeding of MSCs and EPCs onto the graft, followed by in situ transplantation.
- Post-transplantation evaluation using CT scans, bronchoscopy, scanning electron microscopy, and immunofluorescence.
Main Results:
- The PCL/dtECM graft demonstrated excellent biomechanical properties and enhanced cell adhesion.
- Post-transplantation, successful neovascularization and epithelialization with abundant cilia were observed.
- CT scans confirmed a patent luminal space and bronchoscopy showed successful anastomosis.
- The combined use of EPCs and MSCs significantly promoted graft vascularization and epithelialization.
Conclusions:
- The developed biomimetic tracheal graft effectively supports vascularization and epithelialization.
- The PCL/dtECM scaffold combined with cell therapy promotes successful tracheal graft integration.
- This innovative approach ensures long-term graft survival and functional recovery in transplantation models.
