[Titration of the hemolytic activity of human complement after radial diffusion]

Area of Science:

• Immunology and Biochemistry

Context:

• Complement system's role in innate immunity
• Hemolytic activity assays

Purpose:

• Quantify human complement hemolytic activity
• Establish a dose-response relationship

Summary:

• Evaluated human complement hemolytic activity using agarose diffusion assay with sensitized erythrocytes.
• Observed a linear correlation between the area of hemolysis and the logarithm of complement concentration.

Impact:

• Provides a quantitative method for assessing complement function.
• Useful for research and diagnostics involving the complement system.