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Updated: May 4, 2026
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
Published on: November 29, 2018
Protocol for the establishment and characterization of lung tumoroids from surgical resection without the use of
Federico Donà1, Silvia Erratico1, Luigi Maria Terracciano2
1IRCCS Humanitas Research Hospital, Via Manzoni 56, Rozzano, 20089 Milan, Italy.
Abstract:
Organoids are vital for studying cancer mechanisms and treatment resistance, but their growth usually depends on costly, animal-derived Matrigel. Here, we present a protocol for expanding and maintaining lung-derived organoids without the need for Matrigel. We describe steps for establishing lung cancer organoids, staining organoids for immunohistochemistry, and cultivating them with ClinoStar. By eliminating dependence on Matrigel and its associated costs, this protocol enhances accessibility and scalability, making organoid-based research more feasible for cancer studies and therapeutic development.
