Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Analyzers: Common Types

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of Obstetrics Simulator in Developing Knowledge and Skills of Managing Obstetrics Emergencies in MBBS Interns.

Journal of obstetrics and gynaecology of India·2026

Same author

Correction to "Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Spectroscopy".

Nano letters·2026

Same author

Wavefront estimation through structured detection in laser scanning microscopy.

Biomedical optics express·2026

Same author

Out-of-pocket expenditure in patients with isolated traumatic brain injury: a two center cohort study, a preliminary report.

Cost effectiveness and resource allocation : C/E·2026

Same author

The role of RNA in the nanoscale organization of α-synuclein phase separation.

NAR molecular medicine·2025

Same author

Structured detection for simultaneous super-resolution and optical sectioning in laser scanning microscopy.

Nature photonics·2025

Same journal

Bi-layer photonic random meta-composite for cryogenic thermal control by ultra-broadband scattering matched reflectance.

Light, science & applications·2026

Same journal

Interferometric scattering for optical tomoslicing of transparent solids.

Light, science & applications·2026

Same journal

Multi-dimensional spatial-temporal projection ultrafast compressed imaging.

Light, science & applications·2026

Same journal

Expanded field of view light-field extended-reality displays with metalens array.

Light, science & applications·2026

Same journal

Experimental observation of counter-intuitive features of photonic bunching.

Light, science & applications·2026

Same journal

High-speed and high-sensitivity multi-gas detection based on parallel heterodyne LITES sensor.

Light, science & applications·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 17, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Array detection enables large localization range for simple and robust MINFLUX.

Eli Slenders¹, Sanket Patil^1,2, Marcus Oliver Held¹

¹Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy.

Light, Science & Applications

|July 3, 2025

Summary

This summary is machine-generated.

This study introduces ISM-FLUX, a new method for single-molecule localization microscopy (SMLM). ISM-FLUX simplifies MINFLUX (Multiple Instruction, Multiple Data) localization, improving its range and ease of use.

More Related Videos

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Related Experiment Videos

Last Updated: Sep 17, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Area of Science:

Super-resolution microscopy
Nanoscale imaging
Biophysics

Background:

MINFLUX (Minimum Information, Maximum Resolution) enhances single-molecule localization microscopy (SMLM) beyond photon count limits.
Conventional MINFLUX uses iterative scanning, which can be complex and prone to errors.

Purpose of the Study:

To introduce ISM-FLUX, a novel MINFLUX implementation using image-scanning microscopy (ISM).
To improve MINFLUX localization precision, range, and system simplicity.

Main Methods:

Developed ISM-FLUX by integrating ISM with a single-photon avalanche diode array detector.
Utilized a scanning strategy that provides precise localization within the trajectory and conventional uncertainty outside.

Main Results:

ISM-FLUX achieves robust localization with a larger effective localization range compared to standard MINFLUX.
The new method simplifies the MINFLUX architecture, reducing system complexity.

Conclusions:

ISM-FLUX offers a more robust and simpler approach to MINFLUX super-resolution microscopy.
This advancement has the potential to increase the accessibility and adoption of MINFLUX technology.