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A versatile and open source design for one and two photon light sheet microscopy.

Antoine Hubert1, Thomas Panier1,2, Geoffrey Migault1

  • 1Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratoire Jean Perrin (LJP), Paris, France.

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Summary
This summary is machine-generated.

We present an open-source design to convert a standard brightfield microscope into a cost-effective two-photon light sheet system. This accessible technology enhances biological imaging capabilities without compromising resolution or speed.

Keywords:
Hollow core fiberLight-sheet microscopyNegative curvature fiberOpen sourceTwo-photon microscopy

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Area of Science:

  • Biophotonics and advanced microscopy techniques.

Background:

  • Two-photon light sheet microscopy (TP-LSM) is powerful for biological imaging.
  • High costs, complexity, and integration issues limit TP-LSM adoption.

Purpose of the Study:

  • To provide an open-source design for an affordable and adaptable TP-LSM system.
  • To enable the transformation of existing brightfield microscopes into advanced light sheet setups.

Main Methods:

  • Developed a detailed open-source design for microscope modification.
  • Integrated a broadband hollow core fiber for simultaneous visible and pulsed laser use.
  • Focused on simplifying construction, alignment, and reducing overall cost.

Main Results:

  • Successfully created a versatile one- and two-photon light sheet microscopy system.
  • Achieved uncompromised image resolution and speed through innovative laser integration.
  • Significantly reduced the financial and technical barriers to implementing TP-LSM.

Conclusions:

  • The open-source design democratizes access to advanced light sheet microscopy.
  • This innovation facilitates broader application of TP-LSM in biological research.
  • The system offers a cost-effective solution for high-performance biological imaging.