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Updated: Sep 17, 2025

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
Published on: April 19, 2019
Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis
Penghui Miao1, Xuanlin Li1, Yongshan Wan1
1College of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, China.
Background:
Cultivated peanut (Arachis hypogaea L.) is a major oil and economic crop. Pod size is one of the important agronomic traits of peanut variety, with a direct impact on peanut yield.
Methods:
In a previous study, AhRUVBL2 was identified by map-based cloning technology as a candidate gene that regulates peanut pod size.
Results:
Overexpression of AhRUVBL2 in transgenic Arabidopsis significantly increased plant height, branch number, leaf size, silique size, seed size, and thousand-seed weight. Further examination revealed an increase in the area of silique exocarp cells, and the number and area of endocarp lignified cells. A total of 337 differentially expressed genes, including PRX (Periaxin) , SAUR (Small Auxin-up RNA), and PYL (Pyrabactin Resistance 1-like), were identified by transcriptome analysis of transgenic Arabidopsis silique. proAhRUVBL2-GUS was found to be expressed explicitly in seeds, and the expression activity of proAhRUVBL2-D893 was significantly greater than that of proAhRUVBL2-79266. Exogenous ABA (abscisic acid) and IAA (indole acetic acid) treatment of proAhRUVBL2-GUS-transformed tobacco leaves revealed that the AhRUVBL2 promoter was hormone-responsive.
Conclusions:
This study sheds light on the function of AhRUVBL2 in regulating plant growth and development. Moreover, characterization of the AhRUVBL2 promoter provides a valuable genetic resource for enhancing peanut yield.
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