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Related Concept Videos

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
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Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
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As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
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The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
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Updated: Sep 17, 2025

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
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Ligation-induced DNA self-assembly.

Qishu Zhang1,2, Zhengyang Sun1,2, Yaduo Wang1,2

  • 1School of Life Sciences, Tsinghua University, Beijing 100084, China.

Nucleic Acids Research
|July 4, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel ligation method for DNA self-assembly. This technique uses transient DNA pairing, which becomes permanent through ligation, enabling the creation of complex DNA nanostructures.

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • DNA ligation is typically used to stabilize pre-formed nanostructures.
  • Self-assembly of DNA nanostructures often relies on stable base pairing.

Purpose of the Study:

  • To utilize DNA ligation as an active driver of self-assembly, rather than a post-assembly stabilization method.
  • To explore the use of transient base pairing in conjunction with ligation for DNA nanostructure formation.

Main Methods:

  • Designing DNA constructs with transiently paired segments.
  • Applying DNA ligation to convert transient base pairs into permanent bonds, thereby inducing self-assembly.
  • Testing the method on various assembly tasks, including discrete and extended nanostructures.

Main Results:

  • Successful construction of discrete and extended DNA nanostructures using the ligation-driven self-assembly method.
  • Demonstration of the method's efficacy in hierarchical assembly, creating higher-order superstructures from preformed DNA nanostructure units.

Conclusions:

  • Ligation can be effectively employed as an active mechanism to drive DNA self-assembly.
  • This approach expands the toolkit for constructing complex and hierarchical DNA nanostructures.