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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Updated: Sep 16, 2025

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
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Condensin II activation by M18BP1.

Alessandro Borsellini1, Duccio Conti2, Erin E Cutts3

  • 1Human Technopole, Milan, Italy.

Molecular Cell
|July 4, 2025
PubMed
Summary
This summary is machine-generated.

MIS18-binding protein 1 (M18BP1) is essential for condensin II

Keywords:
AlphA Fold IIM18BP1SMC complexescentromereschromosome condensationcondensin IIcrosslinking mass spectrometrycryo-EMhaploid geneticsmitosis

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Condensin complexes are crucial for genome organization during mitosis.
  • Condensin II initiates chromosome condensation, but its activation and chromatin loading mechanisms are not fully understood.

Purpose of the Study:

  • To identify the factor responsible for condensin II's localization to chromatin at mitotic entry.
  • To elucidate the regulatory mechanism controlling condensin II activity during the cell cycle.

Main Methods:

  • Genetic screening and proteomic analysis were employed.
  • Protein-protein interaction studies were conducted.

Main Results:

  • MIS18-binding protein 1 (M18BP1) was identified as essential for condensin II chromatin localization.
  • M18BP1 directly binds to the CAP-G2 subunit of condensin II.
  • MCPH1, an antagonist of condensin II, competes with M18BP1 for CAP-G2 binding during interphase.

Conclusions:

  • A switch from MCPH1 to M18BP1 binding at mitosis onset activates condensin II.
  • This M18BP1-dependent activation of condensin II drives chromosome condensation.
  • Regulation of the M18BP1-condensin interaction governs genome condensation states in interphase and mitosis.