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Isolation and characterization of Pseudomonas phage HJ01 and its therapeutic efficacy in canine pyoderma

Yixian Wei1, Ziyong Li1, Jianlong Lao1

  • 1College of Animal Science and Technology, Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi University, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, Guangxi, 530004, China.

BMC Veterinary Research
|July 5, 2025
PubMed
Abstract

Insights

Phage therapy using HJ01 shows promise for treating multidrug-resistant Pseudomonas aeruginosa in dogs with pyoderma. This approach offers an alternative to antibiotics, reducing resistance and public health risks.

Area of Science:

  • Veterinary Medicine
  • Microbiology
  • Bacteriophage Therapy

Background:

  • Pseudomonas aeruginosa is a leading cause of canine pyoderma.
  • Antibiotic resistance in P. aeruginosa necessitates novel therapeutic strategies.
  • Phage therapy presents a potential alternative to conventional antibiotics.

Purpose of the Study:

  • To isolate and characterize a lytic phage effective against multidrug-resistant P. aeruginosa.
  • To evaluate the therapeutic efficacy of the isolated phage (HJ01) in a canine pyoderma model.

Main Methods:

  • Isolation of phage HJ01 from wastewater.
  • Genomic and biological characterization of phage HJ01.
  • In vivo efficacy testing of HJ01 in a P. aeruginosa-induced canine pyoderma model.

Main Results:

  • Phage HJ01 demonstrated optimal activity within a specific pH and temperature range.
  • HJ01 significantly reduced clinical signs of pyoderma, including pruritus and skin lesions.
  • Histopathology revealed accelerated epidermal repair and reduced inflammation in treated dogs.

Conclusions:

  • Phage HJ01 is a viable therapeutic candidate for canine pyoderma caused by multidrug-resistant P. aeruginosa.
  • This study establishes phage therapy as a novel treatment for canine pyoderma.
  • The findings support phage therapy's role in combating antibiotic resistance and zoonotic risks.
Keywords:
Pseudomonas aeruginosaCanineMulti-drug resistant phage therapyPyoderma

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