Exploring plant-based approaches: A review of protein extraction, characterization, food and pharmacological...

Protein is an essential macronutrient for human growth and survival. As global demand rises, sustainable and unconventional protein sources with high nutritional and functional potential must be explored, though many remain underutilized and overlooked. Among them, fruit pulp proteins offer a promising and sustainable alternative with unique bioactive properties and diverse applications in the food industry. This review addresses various methods for extracting proteins from fruit pulp, including chemical, salt, alkaline, enzymatic, ultrasound-assisted, microwave-assisted, high-voltage electrical discharge, and reverse micelle extraction. Furthermore, it explores the chemistry and characterization of fruit pulp proteins, which is crucial for understanding their functional properties and the diverse applications of fruit pulp proteins as potential substitutes for animal-derived proteins in food and pharmaceuticals. Overall, the exploration and integration of fruit pulp proteins into mainstream food production can contribute to a more resilient and diversified protein supply, addressing both nutritional and ecological concerns by focusing future research.