Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Bacterial Membrane Vesicles on Cellular Responses in Leishmania amazonensis-Infected Macrophages In Vitro.

Scandinavian journal of immunology·2026
Same author

Bioorthogonally reinforced injectable granular hydrogels synergizing ECM mimicry with microporosity for skin tissue engineering.

Biomaterials science·2026
Same author

Modeling cancer with bacteria-integrated tumor microenvironments using biomaterials: Emerging concepts and opportunities.

Materials today. Bio·2026
Same author

Injectable Silk Fibroin-Puerarin Hydrogels with Tunable Supramolecular Organization as a Potential Platform for Tissue Engineering.

ACS omega·2026
Same author

Targeting Glutamate Excitotoxicity With Memantine Modulates Glial Response and Protects Motoneurons After Spinal Root Lesion.

Journal of neurochemistry·2026
Same author

Anti-Inflammatory and Synaptic Protective Effects of TNF-α Inactivation in the MDX Mouse Model.

Current issues in molecular biology·2026

Related Experiment Video

Updated: Sep 16, 2025

Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap
08:05

Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap

Published on: November 3, 2017

7.1K

Long-Gap Sciatic Nerve Regeneration Using 3D-Printed Nerve Conduits with Controlled FGF-2 Release.

Diego N Rodriguez-Sanchez1,2, Leticia A M de Carvalho1, Ingri Mancilla-Corzo3

  • 1Laboratory of Nerve Regeneration, Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Sao Paulo 13083-970, Brazil.

ACS Applied Materials & Interfaces
|July 7, 2025
PubMed
Summary
This summary is machine-generated.

This study developed 3D-printed nerve guidance conduits (NGCs) using PCL and GelMA with FGF-2 to treat peripheral nerve injuries. These bioactive NGCs significantly improved nerve regeneration and functional recovery in rats.

Keywords:
3D printingFGF-2biomaterialsgelatin methacryloylnerve guidance conduitsperipheral nerve regenerationpolycaprolactone

More Related Videos

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
09:19

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Published on: December 8, 2017

14.9K
Implantation and Control of Wireless, Battery-free Systems for Peripheral Nerve Interfacing
07:13

Implantation and Control of Wireless, Battery-free Systems for Peripheral Nerve Interfacing

Published on: October 20, 2021

3.4K

Related Experiment Videos

Last Updated: Sep 16, 2025

Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap
08:05

Transplantation of Schwann Cells Inside PVDF-TrFE Conduits to Bridge Transected Rat Spinal Cord Stumps to Promote Axon Regeneration Across the Gap

Published on: November 3, 2017

7.1K
Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
09:19

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

Published on: December 8, 2017

14.9K
Implantation and Control of Wireless, Battery-free Systems for Peripheral Nerve Interfacing
07:13

Implantation and Control of Wireless, Battery-free Systems for Peripheral Nerve Interfacing

Published on: October 20, 2021

3.4K

Area of Science:

  • Biomaterials Engineering
  • Regenerative Medicine
  • Neuroscience

Background:

  • Peripheral nerve injuries (PNIs) often result in significant sensory and motor deficits.
  • Current reconstructive surgeries for PNIs have variable outcomes.
  • Three-dimensional (3D) printing offers a platform for creating advanced nerve guidance conduits (NGCs).

Purpose of the Study:

  • To develop and evaluate 3D-printed NGCs using polycaprolactone (PCL) and gelatin methacryloyl (GelMA) for peripheral nerve repair.
  • To incorporate fibroblast growth factor 2 (FGF-2) for enhanced neurotrophic support and controlled release.
  • To assess the efficacy of these bioactive NGCs in a rat model of long-gap peripheral nerve injury.

Main Methods:

  • Fabrication of NGCs using a 3D printing process with PCL and GelMA (10% w/v).
  • Integration and controlled release of thermostable fibroblast growth factor 2 (FGF-2) within the GelMA matrix.
  • In vitro assessment of cell viability, proliferation, and gene expression (Schwann cells, MSCs).
  • In vivo implantation of NGCs in a rat model of long-gap peripheral nerve injury, followed by functional, electrophysiological, and histological analysis at 4 and 12 weeks.

Main Results:

  • Optimized GelMA concentration (10% w/v) ensured excellent printing fidelity, mechanical properties, and rheology.
  • FGF-2 incorporation led to sustained release over 30 days, enhanced cell metabolism, and promoted vascularization-related gene expression in MSCs.
  • NGC implantation significantly improved sensory and motor recovery, electrophysiological function, and nerve regeneration in rats at 12 weeks.
  • Early signs of regeneration at 4 weeks included Schwann cell proliferation, P75NTR expression, myelination, and neurofilament organization.

Conclusions:

  • 3D-printed NGCs composed of PCL and GelMA, functionalized with FGF-2, are biocompatible and promote nerve regeneration.
  • These bioactive NGCs demonstrate significant therapeutic potential for repairing long-gap peripheral nerve injuries.
  • The developed NGCs represent a promising alternative to traditional nerve autografts.