Related Experiment Video

Updated: Sep 16, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.2K

Genome assembly of Acinetobacter baumannii strain 1432 carrying KL130 and eight prophage regions

Anastasia S Sukhova1, Yury P Skryabin1, Peter V Evseev2

  • 1State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia.

Microbiology Resource Announcements
|July 7, 2025
PubMed

Insights

This study presents the genome assembly of Acinetobacter baumannii strain 1432, a host for phage vB_AbaP_AS12. The bacterial genome contains a capsule biosynthesis locus and eight predicted prophage regions.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriology

Background:

  • Acinetobacter baumannii is an opportunistic pathogen.
  • Phage-host interactions are crucial in microbial ecology and evolution.
  • Understanding bacterial genomes aids in developing novel therapeutic strategies.

Purpose of the Study:

  • To perform and report the genome assembly of Acinetobacter baumannii strain 1432.
  • To identify key genomic features of the strain, including phage integration sites and capsule biosynthesis loci.

Main Methods:

  • Whole-genome sequencing and assembly.
  • Bioinformatic analysis for prophage prediction.
  • Identification of specific genetic loci, such as capsule biosynthesis genes.

Main Results:

  • Genome assembly of Acinetobacter baumannii strain 1432 reported.
  • Strain identified as the host for phage vB_AbaP_AS12.
  • Capsule biosynthesis locus KL130 and eight prophage regions detected.

Conclusions:

  • The genome assembly provides a valuable resource for studying Acinetobacter baumannii.
  • The presence of specific loci offers insights into bacterial adaptation and phage interactions.
  • Further research can leverage this genomic data for understanding strain 1432 and its associated phage.
Keywords:
Acinetobacter baumanniigenome assembly

More Related Videos

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

534
Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

744

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
19.3K
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
160
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.5K