Peptide-Enhanced Bioactive Hydrogel Combined with Photodynamic-Phage Synergistic Antibacterial Therapy System...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Infectious Diseases

Background:

• Bacterial resistance presents a significant clinical challenge, necessitating innovative therapeutic strategies.
• Effective wound healing requires addressing bacterial infection and promoting tissue regeneration.

Purpose of the Study:

• To design and evaluate a peptide-enhanced bioactive hydrogel with a synergistic photodynamic-phage antibacterial effect.
• To investigate the hydrogel's efficacy in promoting wound healing and explore its underlying molecular mechanisms.

Main Methods:

• Fabrication and characterization of LaFeO3@C3N4 nanocomposite and peptide-modified hydrogel using techniques like XRD, XPS, SEM, FT-IR, and 1HNMR.
• Assessment of synergistic antibacterial activity and wound healing effects in vitro and in vivo.
• Exploration of molecular mechanisms of tissue regeneration via transcriptomic and protein expression analysis.

Main Results:

• The LaFeO3@C3N4 nanocomposite demonstrated photodynamic properties producing reactive oxygen species (ROS).
• The peptide-enhanced hydrogel exhibited synergistic antibacterial effects and promoted wound healing.
• The therapy system (QBC@DP-P-phi) significantly enhanced cell proliferation and migration by regulating PI3K-Akt, VEGF, and MAPK signaling pathways.

Conclusions:

• The peptide-enhanced bioactive hydrogel with photodynamic-phage synergistic therapy is a promising candidate for treating infected wounds.
• This novel system effectively combats bacterial resistance and facilitates tissue regeneration.
• The study elucidates the molecular pathways involved in the therapeutic effects, paving the way for clinical applications.