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Barriers in cardiac substrate supply.

H Kammermeier, B Wein, P Gerards

    Basic Research in Cardiology
    |January 1, 1985
    PubMed
    Summary
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    Investigating heart capillary transfer reveals variable resistance, particularly for glucose and lactate. Further studies using isolated cardiac myocytes clarified sarcolemmal transfer mechanisms for these substances.

    Area of Science:

    • Cardiovascular Physiology
    • Cellular Metabolism

    Background:

    • Capillary walls create concentration gradients between vascular and interstitial spaces for heart-related substances.
    • Diffusional resistance in isolated hearts is variable, influenced by insulin for glucose and concentration/direction for lactate.

    Purpose of the Study:

    • To investigate sarcolemmal (cell membrane) transfer mechanisms for glucose and lactate in cardiac tissue.
    • To characterize the factors influencing the transport of glucose and lactate across the cardiac myocyte membrane.

    Main Methods:

    • Utilized isolated cardiac myocytes to overcome the unpredictable interstitial concentrations found in whole hearts.
    • Studied the sarcolemmal transfer of glucose and lactate under various conditions.

    Main Results:

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    • Lactate transfer exhibited saturation kinetics.
    • Lactate transfer was dependent on pyruvate concentration, showing inhibition at low and enhancement at high levels.
    • Lactate transfer increased linearly as pH decreased from 8.0 to 6.7.

    Conclusions:

    • Sarcolemmal transfer of glucose and lactate can be studied effectively in isolated cardiac myocytes.
    • Lactate transport is complex, influenced by substrate concentration, pyruvate, and pH, suggesting intricate regulatory mechanisms.