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Updated: Sep 16, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Oral microbiome dynamics in Postmortem Interval estimation: research standards and guidelines.

Aleksandra Haenel¹, Tomasz Grzybowski², Katarzyna Skonieczna¹

¹Department of Forensic Medicine, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, Poland.

Archiwum Medycyny Sadowej I Kryminologii

|July 9, 2025

Summary

This summary is machine-generated.

Estimating the time of death using oral microbiome analysis shows promise for forensic science. Standardizing methods can improve accuracy in determining the postmortem interval (PMI).

Keywords:

PMI Postmortem Interval oral microbiome

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Area of Science:

Forensic Science
Microbiology
Genetics

Background:

Postmortem interval (PMI) estimation is vital in forensic investigations but challenging due to decomposition variability.
Current methods for PMI determination rely heavily on expert experience and are prone to inaccuracies.
Gut microbiome analysis has emerged as a potential tool for PMI estimation.

Purpose of the Study:

To review existing literature on oral microbiome profiling for PMI estimation.
To identify methodological limitations hindering the scientific value of current oral microbiome studies.
To propose research standards and guidelines for oral microbiome analysis in forensic contexts.

Main Methods:

Literature review of published studies on oral microbiome and postmortem interval.
Analysis of methodological approaches and limitations in existing research.
Synthesis of findings to recommend standardization for future studies.

Main Results:

Emerging evidence suggests oral microbiome profiling can aid in PMI estimation.
Significant methodological limitations currently reduce the reliability of oral microbiome data for forensic use.
A need for standardized protocols is evident to enhance accuracy and reproducibility.

Conclusions:

Standardizing oral microbiome research is crucial for developing reliable methods for PMI determination.
Implementing standardized oral microbiome studies could lead to innovative forensic genetics practices.
Enhanced accuracy in PMI estimation through oral microbiome analysis can significantly aid investigations.