Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A general method of deriving the best binding site model consistent with experimental binding data.

J B Pedersen, S M Pedersen

    Biophysical Chemistry
    |October 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Reduction of calprotectin and phosphate during testosterone therapy in aging men: a randomized controlled trial.

    Journal of endocrinological investigation·2016
    Same author

    Cost-Effectiveness Analysis of Surface Flow Constructed Wetlands (SFCW) for Nutrient Reduction in Drainage Discharge from Agricultural Fields in Denmark.

    Environmental management·2015
    Same author

    Cerebral energy metabolism during induced mitochondrial dysfunction.

    Acta anaesthesiologica Scandinavica·2012
    Same author

    Analysis of lipid peroxidation kinetics. 1. Role of recombination of alkyl and peroxyl radicals.

    The journal of physical chemistry. B·2008
    Same author

    Singlet and triplet products of the geminate recombination of a radical pair with a single magnetic nucleus (I = 1/2).

    The journal of physical chemistry. A·2005
    Same author

    Gastro-esophageal reflux demonstrated by radiography in infants less than 1 year of age. Comparison with pH monitoring.

    Acta radiologica (Stockholm, Sweden : 1987)·2003
    Same journal

    Machine learning-driven identification of pan-PI3K inhibitors: A hybrid virtual screening approach combining naïve Bayesian classification, pharmacophore modeling, and consensus scoring-based molecular docking.

    Biophysical chemistry·2026
    Same journal

    Optimizing grid preparation methods for TEM imaging of amyloid-forming proteins.

    Biophysical chemistry·2026
    Same journal

    Biogenic reduction mechanisms in iron oxide nanoparticle synthesis: Strategies to mitigate microbial resistance.

    Biophysical chemistry·2026
    Same journal

    Novel Pennisetum Alopecuroides-derived activated carbon for high-efficiency Tartrazine Removal: Box-Behnken optimization and DFT-assisted mechanistic insights.

    Biophysical chemistry·2026
    Same journal

    Reactive molecular dynamics investigation of the first steps of coronavirus (COVID-19) viral-protein ligands fragment (SARS-CoV-2).

    Biophysical chemistry·2026
    Same journal

    Probing the interactions between bovine hemoglobin and three berberine saturated fatty acid salts by multi-spectral techniques, conductimetry and molecular docking.

    Biophysical chemistry·2026
    See all related articles

    This study presents a novel analysis of binding data to determine the best molecular binding site models. The method accurately quanties the number and interactions of binding sites from experimental data.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Biophysics

    Background:

    • Understanding molecular interactions is crucial in biochemistry.
    • Characterizing binding sites informs drug design and biological pathway analysis.
    • Existing models may not fully capture complex binding behaviors.

    Purpose of the Study:

    • To develop a robust method for analyzing binding data.
    • To determine the optimal binding site model from experimental results.
    • To quantify key parameters of molecular interactions.

    Main Methods:

    • Analysis of experimental binding data.
    • Construction and root analysis of a binding polynomial.
    • Fitting of Adair constants to derive interaction parameters.

    Related Experiment Videos

    Main Results:

    • Identification of the best binding site model.
    • Quantification of independent and interacting binding sites (dimers, tetramers).
    • Determination of intrinsic association constants and degree of interaction.

    Conclusions:

    • The developed analysis provides a comprehensive model for homotropic binding.
    • This method accurately characterizes binding site stoichiometry and affinity.
    • The findings offer valuable insights into molecular recognition processes.