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styMdtM gene deletion with modified λ Red recombineering approach: Implications for multidrug resistance in
Anam Tariq1, Aqsa Shaheen2, Mazhar Iqbal1
1Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Punjab, Pakistan.
Journal of Microbiological Methods
|July 10, 2025
Abstract:
styMdtM is a multidrug efflux transporter of Salmonella Typhi. In the present study, a modified λ Red recombineering approach using shorter flanking arms (ca. 50 bp) was used to generate an styMdtM gene deletion in the S. Typhi genome. This approach worked efficiently to knockout the gene and the ensuing S. Typhi strain was considerably more sensitive to antibiotics.
Keywords:
Efflux pumpGene knockoutLambda Red recombineeringMultidrug resistanceSalmonella TyphistyMdtM
