Transcriptomic insights into reduced nitrous oxide emissions in one-stage partial nitritation/anammox process exposed
Chenxi Zeng1, Zhenguo Chen1, Junxing Liu1
1Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, School of Environment, South China Normal University, Guangzhou 510006, PR China; SCNU (NAN' AN) Green and Low-carbon Innovation Center & Guangdong Provincial Engineering Research Center of Intelligent Low-carbon Pollution Prevention and Digital Technology, South China Normal University, Guangzhou 510006, PR China.
None:
The partial nitritation/anammox (PN/A) process is an innovative and efficient nitrogen removal technology for wastewater treatment, but the potential nitrous oxide (N2O) emissions may hinder its application due to climate concerns. This study focused on N2O emissions and related microbial mechanisms in one-stage PN/A process under long-term 0.5 mg/L sulfamethazine (SMZ) stress. Compared to control reactor (0 mg/L SMZ), such SMZ stress reduced N2O emission factor by 24 % with nitrogen removal performance unaffected (p > 0.05). Transcriptome analysis revealed the transcript levels of hao and norBC were down-regulated by 80.28 % and 79.73 %, while nosZ was up-regulated by 7.97 %, indicating SMZ stress reduces N2O emissions by inhibiting both hydroxylamine oxidation and denitrification while enhancing N2O reduction. These findings provide a scientific foundation for understanding the characteristics and mechanisms of N2O emissions in one-stage PN/A systems under antibiotic stress, promoting its application for reducing greenhouse gas emissions in practical wastewater treatment processes.
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