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Analytical patterns and biochemical properties of macro creatine kinase type 2
Clinical Chemistry
|December 1, 1985
Summary
Macro creatine kinase (CK) type 2, a variant found in patient sera, complicates diagnostic tests. This heat-stable, high-molecular-weight form originates from mitochondria, not cytoplasm, impacting creatine kinase-MB assays.
Area of Science:
- Clinical Chemistry
- Biochemistry
Background:
- Macro creatine kinase (CK) type 2 is a variant found in patient sera.
- Its presence can interfere with standard diagnostic assays for CK isoenzymes.
Purpose of the Study:
- To characterize macro CK type 2.
- To understand its interference mechanisms in CK-MB assays.
Main Methods:
- Exclusion chromatography was used to confirm macro CK type 2 in 105 patient sera.
- Electrophoresis, ion-exchange chromatography, and immunoinhibition were employed to analyze CK isoenzymes.
Main Results:
- Macro CK type 2 exhibits distinct patterns based on assay methodology, complicating CK-MB interpretation.
- This variant is heat-stable, has increased molecular mass, and high energy of activation, unlike cytoplasmic CK isoenzymes.
Conclusions:
- Macro CK type 2 is not of cytoplasmic origin but represents a distinct serum CK activity.
- Mitochondrial CK released during severe cell damage typically appears as macro CK type 2 in blood.