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Curtain Flow Column: Optimization of Efficiency and Sensitivity

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Published on: June 12, 2016

A universal separation quality factor (SQF) for evaluating liquid chromatographic separations.

Szabolcs Fekete¹, Róbert Kormány², Mateusz Imiołek¹

¹Waters Corporation, 1211 Geneva, Switzerland.

Journal of Chromatography. A

|July 11, 2025

Summary

A new separation quality factor (SQF) combines multiple metrics into one score for evaluating liquid chromatography performance. This provides a comprehensive and objective measure for method development and comparison.

Keywords:

Column comparison Critical peak pair Method development Peak distribution Performance metrics

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Area of Science:

Analytical Chemistry
Chromatography Science

Background:

Traditional liquid chromatography (LC) assessments use individual metrics like plate number and peak symmetry.
Existing metrics lack a comprehensive, normalized score for overall separation quality.

Purpose of the Study:

Introduce a novel Separation Quality Factor (SQF) for robust evaluation of chromatographic separations.
Develop a single, normalized score integrating multiple sub-metrics for LC performance.

Main Methods:

Developed a Separation Quality Factor (SQF) by integrating five distinct sub-metrics.
Normalized each sub-metric to a dimensionless value between 0 and 1.

Main Results:

The SQF provides a single, normalized value representing overall separation quality.
The SQF offers a robust, objective, and scalable method for evaluating chromatographic separations.

Conclusions:

The SQF enhances the ability to compare different chromatographic separations objectively.
SQF facilitates improved optimization strategies in chromatographic method development.