Related Experiment Video
Updated: Jul 18, 2026

10:35
Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
Published on: February 10, 2018
33.8K
Long Shelf-Life Ready-to-Eat Plant-Based Whole Hard-Boiled Eggs: Low Allergenic and Regular Formulas
Kanda Wongwailikhit1, Suvimol Soithongsuk2, Yupakanit Puangwerakul3
1Department of Chemistry, Faculty of Science, Rangsit University, Pathum Thani 12000, Thailand.
Foods (Basel, Switzerland)
|July 12, 2025
Summary
Researchers developed a shelf-stable, plant-based hard-boiled egg alternative using mung bean and wheat proteins. Optimal preservation via thermal pasteurization or gamma irradiation ensures consumer acceptance and extended shelf life.
Area of Science:
- Food Science and Technology
- Plant-Based Foods
- Food Preservation
Background:
- Growing demand for plant-based alternatives to traditional animal products.
- Need for shelf-stable, convenient, and allergen-friendly egg substitutes.
- Challenges in replicating the sensory and structural properties of whole eggs using plant proteins.
Purpose of the Study:
- To develop a shelf-stable, plant-based whole hard-boiled egg analogue.
- To evaluate different plant proteins for optimal sensory and visual characteristics.
- To determine effective preservation methods for extending shelf life at ambient temperatures.
Main Methods:
- Formulation of egg white and yolk components using six plant proteins (soy, mung bean, pea, rice, potato, wheat).
- Application of thermal pasteurization (75-85 °C) and gamma irradiation (2-5 kGy) for preservation.
- Sensory evaluation by 100 panelists and accelerated shelf life testing with Q10 modeling.
Main Results:
- Mung bean and wheat proteins yielded the most promising sensory and visual qualities.
- Optimal preservation conditions identified as 75 °C for 15 min (thermal) and 3.5 kGy (gamma irradiation).
- Sensory evaluation showed favorable acceptance for appearance, texture, and overall liking.
- Predicted shelf life of 188 days (thermal) and 253 days (gamma irradiation) at 30 °C.
Conclusions:
- A consumer-acceptable, plant-based hard-boiled egg analogue is feasible.
- Mung bean and wheat proteins are suitable for developing egg analogues.
- Thermal pasteurization and gamma irradiation effectively extend shelf life while maintaining quality.
Related Concept Videos
Proteins: Dietary Sources and Requirements
Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...
Lipids: Dietary Sources and Requirements
Lipids are an essential component of a balanced human diet. Triglycerides, which make up the majority of dietary lipids, are found in both saturated fats—commonly present in meat, dairy products, and certain tropical plants like coconut, and hydrogenated oils such as margarine and baking shortenings (trans fats)—and unsaturated fats, which are abundant in seeds, nuts, olive oil, and most vegetable oils. The main sources of cholesterol include egg yolks, various meats and organ meats, shellfish,...
Pasteurization and Food Preservation
Pasteurization is a widely employed thermal processing technique designed to enhance the safety and shelf life of perishable food and beverages. By subjecting products to specific high temperatures for controlled durations, this method effectively inactivates pathogenic microorganisms and spoilage enzymes without significantly compromising sensory qualities. The technique has been pivotal in food safety management, especially for consumables susceptible to microbial contamination such as milk,...

