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Design and Characterization of PAA/CHI/Triclosan Multilayer Films with Long-Term Antibacterial Activity
Balzhan Savdenbekova1,2, Aruzhan Sailau1,2, Ayazhan Seidulayeva1,2
1Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan.
Polymers
|July 12, 2025
Summary
This study developed a stable, pH-responsive antibacterial coating using chitosan, polyacrylic acid, and triclosan for biomedical applications. The coating effectively prevents implant-associated infections by releasing triclosan in acidic environments.
Area of Science:
- Biomaterials Science
- Infectious Disease Prevention
- Surface Chemistry
Background:
- Implant-associated infections (IAIs) pose a significant challenge in biomedical applications.
- Antibacterial coatings are essential for preventing IAIs and improving implant longevity.
- Chitosan (CHI) and polyacrylic acid (PAA) offer biocompatible platforms for drug delivery systems.
Purpose of the Study:
- To design and evaluate a multilayer antibacterial coating incorporating triclosan (TCS).
- To investigate the pH-dependent behavior and stability of the PAA/CHI/TCS coating.
- To assess the antibacterial efficacy and drug release kinetics of the developed coating.
Main Methods:
- Layer-by-layer (LbL) self-assembly technique used for coating fabrication.
- Fourier-transform infrared (FTIR) spectroscopy confirmed triclosan incorporation.
- Atomic force microscopy (AFM) and scanning electron microscopy (SEM) analyzed surface properties.
- Disk diffusion assays evaluated antibacterial activity against *S. aureus* and *E. coli*.
- Long-term stability and pH-dependent drug release kinetics were assessed.
Main Results:
- Coatings assembled at pH 5 (PAA5/CHI5/TCS) showed significant antibacterial activity (60.0 mm for *S. aureus*, 33.67 mm for *E. coli*).
- The coating demonstrated excellent long-term stability, maintaining structural integrity and antimicrobial properties for 40 days.
- Triclosan release was enhanced under acidic conditions (pH 5.5) compared to physiological pH (7.4).
Conclusions:
- The developed multilayer PAA/CHI/TCS coating is a promising candidate for biomedical applications.
- The coating exhibits potent antibacterial activity and sustained drug release properties.
- Its stability and pH-responsive release mechanism offer advantages for preventing implant-associated infections.
