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Updated: Sep 16, 2025
Immunophenotyping of Orthotopic Homograft Syngeneic of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
Published on: October 9, 2018
Multi-Omics Integration of Lactylation- and PANoptosis-Based Signatures in Lung Adenocarcinoma: Prognostic
Zhenhao Xu1, Yisha Huang1, Xiuling Yu1
1MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Abstract:
Lactylation and PANoptosis are emerging modes of tumor progression regulation; however, their interplay and effect on the prognosis for lung adenocarcinoma (LUAD) remain unclear. This research analyzed both bulk and single-cell transcriptomic profiles of LUAD and identified 506 potential markers related to lactylation and PANoptosis. Employing 117 machine learning approaches and 5 LUAD datasets, lactylation and PANoptosis-related signatures (LAPRS) and further predictive nomograms were constructed with 85 prognostic genes. The performance of LAPRS was validated with multifaceted validation, including Kaplan-Meier analysis, time-dependent ROC curves and comparison with 55 existing LUAD models. LAPRS enabled the stratification of LUAD patients into high- and low-risk subgroups. Through additional investigation, high-risk individuals showed elevated genomic alterations, reduced immune infiltration, and poorer immunotherapy response, while low-risk individuals showed better drug sensitivity and a higher tumor mutation burden. Further analysis via 18 models and experimental validation revealed APOL1 as a poor prognostic factor, potentially interacting with the lactylation-related gene VIM through TNF signaling. This research clarifies the mechanistic roles of lactylation and PANoptosis in LUAD and proposes APOL1 as a novel prognostic marker, offering insights for therapeutic stratification.
