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Related Concept Videos

Acne Infection01:27

Acne Infection

98
Acne is a multifactorial skin condition primarily affecting adolescents and young adults, with a global prevalence estimated to exceed 75% in this demographic. The condition is characterized by the formation of comedones (blackheads and whiteheads), papules, pustules, nodules, and, in severe cases, cysts, particularly in areas rich in sebaceous glands such as the face, neck, chest, and back. The pathogenesis involves increased sebum production, follicular hyperkeratinization, colonization by...
98

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Updated: May 5, 2026

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT Cells.

Carolina Manosalva1, Pablo Alarcón2, Lucas Grassau1

  • 1Institute of Pharmacy, Faculty of Sciences, Universidad Austral de Chile, Valdivia 5090000, Chile.

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|July 12, 2025
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Summary

Andrographolide promotes skin healing under photodamage by reducing inflammation and restoring energy metabolism. This bioactive compound aids tissue repair by modulating key inflammatory and metabolic pathways, offering potential therapeutic benefits.

Keywords:
andrographolidekeratinocytemetabolismphotoagingwound healing

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Area of Science:

  • Dermatology and Molecular Biology
  • Natural Product Chemistry
  • Bioenergetics and Metabolism

Background:

  • Andrographolide (AP) possesses anti-inflammatory and antioxidant properties beneficial for wound healing.
  • The impact of AP on energy metabolism during tissue repair and its role in UVB-induced photoaging are not well understood.
  • Photoaging exacerbates wound healing challenges, necessitating research into compounds that address both conditions.

Purpose of the Study:

  • To investigate the multitarget therapeutic effects of Andrographolide on wound healing under photoaging conditions.
  • To elucidate the molecular mechanisms underlying AP's action using network pharmacology and experimental validation.
  • To assess AP's influence on inflammatory and metabolic pathways critical for skin regeneration.

Main Methods:

  • Network pharmacology and bioinformatic analysis to identify key therapeutic targets.
  • In vitro scratch wound assays using UVB-exposed HaCaT cells to evaluate keratinocyte migration.
  • GC/MS-based metabolomics to analyze metabolic pathway alterations.
  • Western blotting to assess protein phosphorylation and expression levels of key inflammatory and metabolic regulators.

Main Results:

  • Andrographolide enhanced keratinocyte migration in UVB-exposed cells.
  • AP suppressed UVB-induced pro-inflammatory gene expression and key inflammatory signaling pathways (ERK1/2, P38).
  • AP modulated metabolic pathways, including fatty acid metabolism, glycolysis, and the TCA cycle, reversing UVB-induced disruptions and enhancing HIF-1α and PPARγ expression.

Conclusions:

  • Andrographolide demonstrates therapeutic potential for wound healing under photoaging by modulating inflammatory and metabolic processes.
  • AP restores metabolic balance essential for tissue regeneration, offering mechanistic insights into skin repair under photodamage.
  • These findings support the development of Andrographolide-based strategies for treating skin photodamage and promoting wound healing.