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Updated: Sep 15, 2025
Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
Protocol for isolating cervico-vaginal fluid cells from Macaca mulatta to study immunological and functional changes
Daniel Short1, Monica Cappelletti2, Anne Vu3
1Department of Metabolism, Digestion and Reproduction, Imperial College London, London SW7 2AZ, UK.
Cervico-vaginal fluid represents an important biological fluid due to its proximity to gestational tissues, such as the cervix, fetal membranes, and myometrium. Here, we present a protocol to isolate cervico-vaginal fluid cells from Macaca mulatta. We describe steps for cervico-vaginal fluid collection, cervico-vaginal cell isolation for nuclear staining and immunophenotyping by flow cytometry, and protein-level detection and quantification by ELISA. This protocol can provide insights into altered immunological changes at the cervico-vaginal interface.
Cervico-vaginal fluid represents an important biological fluid due to its proximity to gestational tissues, such as the cervix, fetal membranes, and myometrium. Here, we present a protocol to isolate cervico-vaginal fluid cells from Macaca mulatta. We describe steps for cervico-vaginal fluid collection, cervico-vaginal cell isolation for nuclear staining and immunophenotyping by flow cytometry, and protein-level detection and quantification by ELISA. This protocol can provide insights into altered immunological changes at the cervico-vaginal interface.
